GeneTaqTM DNA polymerase is a thermostable Taq DNA polymerase that possesses a 5’ →3’ polymerase activity, a 5´→3’ exonuclease activity and a 3’ terminal deoxynucleotidyl transferase activity (addition of single dATP to the duplex DNA). It has been optimized for use in rapid amplification of DNA from trace amounts of starting material, regardless of a template’s GC content.
GeneTaqTM DNA Polymerase with Green Reaction Buffer
The physical purity is greater than 99% as determined by SDS-PAGE.
·Low bacterial DNA contamination
A bacterial TaqMan qPCR assay targeting 16S rRNA was performed with at least 5 ul GeneTaqTM DNA polyperase as a template. The level of DNA contamination in GeneTaqTM DNA polyperase has been estimated at less than 10 genome equivalents of bacterial DNA per unit of the enzyme.
The extension times are generally 30 seconds per kb for complex genomic samples, but can be reduced to 10 seconds per kb for simple templates such as plasmid, bacterial and viral DNA.
· Routine PCR amplification of DNA fragments up to 5 kb
· Generating PCR products with 3'-dA overhangs for TA cloning