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T4 DNA Ligase

BioGeek T4 DNA 连接酶

product Catalog Size Price Download

T4 DNA Ligase

BG10201S

20000 U

¥267.00

Instruction

BG10201L

100000 U

¥1100.00

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. With the optimized reaction buffer, the enzyme can ligate DNA sticky or blunt end fragments as well as repair single-stranded nicks in duplex DNA and some DNA/RNA hybrids.



Features

·High purity

The physical purity is greater than 99% as determined by SDS-PAGE.

·Low bacterial DNA contamination

A bacterial TaqMan qPCR assay targeting 16S rRNA was performed with at least 5 ul BioGeekTM T4 DNA Ligase as a template. The level of DNA contamination in BioGeekTM T4 DNA Ligase has been estimated at less than 10 genome equivalents of bacterial DNA per unit of the enzyme.

·High efficiency

T4 DNA Ligase catalyzes the ligation of sticky or blunt end DNA fragments in only 5 minutes at room temperature


Applications

· Cloning of restriction fragments

· Ligation of PCR fragments

· Repairing single-stranded nicks in duplex DNA, RNA and DNA/RNA hybrids

· DNA circularization

· DNA fragment insertion into vectors

· Golden Gate reactions

· Joining linkers and adapters to blunt-ended DNA for library construction


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