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Quick LigaseTM

BioGeek Quick Ligase

Product Catalog Size Price Download

Quick LigaseTM Kit

BG10202S

30 R

¥600.00

Instruction

BG10202L

150 R

¥2833.00

Description

Quick LigaseTM catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. With the optimized reaction buffer, the enzyme can ligate DNA sticky or blunt end fragments in 5 minutes at room temperature (25 °C).


Features

·High purity

The physical purity is greater than 99% as determined by SDS-PAGE.

·Low bacterial DNA contamination

A bacterial TaqMan qPCR assay targeting 16S rRNA was performed with at least 5 ul BioGeekTM Quick LigaseTM as a template. The level of DNA contamination in BioGeekTM Quick LigaseTM has been estimated at less than 10 genome equivalents of bacterial DNA per unit of the enzyme.

·High efficiency

BioGeekTM Quick LigaseTM catalyzes the ligation of sticky or blunt end DNA fragments in only 5 minutes at room temperature (25 °C).


Applications

· Cloning of restriction fragments

· Ligation of PCR fragments

· Repairing single-stranded nicks in duplex DNA, RNA and DNA/RNA hybrids

· DNA circularization

· DNA fragment insertion into vectors

· Golden Gate reations

· Joining linkers and adapters to blunt-ended DNA for library construction


Order

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