SyngenTech has developed rapid, simple and highly efficient cloning kits for the direct insertion of annealed gRNA oligos into a Cas9 vector. Using these kits, positive clones can easily be obtained within 2 days. To ensure the efficacy of gRNAs, it is advisable to design 3-6 gRNAs against one target.
Shematic diagram for the construction of gRNA-Cas9 vector (Vector construction kit for gene editing):
Shematic diagram for the construction of gRNA-Cas9-KRAB vector (Vector construction kit for transcriptional inhibition):
Shematic diagram for the construction of gRNA-Cas9-VP64 vector (Vector construction kit for transcriptional activation):
SyngenTech also provides custom CRISPR/Cas9 vectors to meet different needs. Custom CRISPR/Cas9 vectors with different combinations of the cis-elements in the following table can be ordered from SyngenTech.
Contact us at 4006803200，or send an email to firstname.lastname@example.org Your questions and requests will be answered by expert staff in SyngenTech within 24 hours.