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Quick cloning kits for CRISPR/Cas9 system

SyngenTech has developed rapid, simple and highly efficient cloning kits for the direct insertion of annealed gRNA oligos into a Cas9 vector. Using these kits, positive clones can easily be obtained within 2 days. To ensure the efficacy of gRNAs, it is advisable to design 3-6 gRNAs against one target.

细胞实验

Shematic diagram for the construction of gRNA-Cas9 vector (Vector construction kit for gene editing):

基因编辑载体构建试剂盒

Shematic diagram for the construction of gRNA-Cas9-KRAB vector (Vector construction kit for transcriptional inhibition):

转录抑制载体构建试剂盒

Shematic diagram for the construction of gRNA-Cas9-VP64 vector (Vector construction kit for transcriptional activation):

转录激活载体构建试剂盒

SyngenTech also provides custom CRISPR/Cas9 vectors to meet different needs. Custom CRISPR/Cas9 vectors with different combinations of the cis-elements in the following table can be ordered from SyngenTech.

图表 gRNA表达载体

Contact us at 4006803200,or send an email to service@syngen.tech Your questions and requests will be answered by expert staff in SyngenTech within 24 hours.