Vector design and construction for the CRISPR/Cas9 system

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas (CRISPR-associated) gene editing technology is a revolutionizing tool developed in recent years. Bacteria use CRISPRs naturally as an anti-viral defense immunity mechanism. In that natural context, CRISPR DNA sequences are transcribed into crRNA (CRISPR RNAs) that in turn base pair with tracrRNA (trans-activating RNA). This tracrRNA/crRNA complex then leads to the destruction of specific viral DNA sequences via the Cas9 nuclease. In the man-made CRISPR/Cas9 system, tracrRNA and crRNA are packaged together to form a single-guide RNA (sgRNA or gRNA) that can guide the Cas9 enzyme to cut at a specific genome locus.

As a dsDNA binding protein guided by RNA, Cas9 endonuclease possesses enormous potential adaptability. For example, a dead (inactive) Cas9 endonuclease can be incorporated to the system to help introduce proteins or nucleic acids to a specific gene region.

SyngenTech also provides custom CRISPR/Cas9 vectors to meet different needs. Custom CRISPR/Cas9 vectors with different combinations of the cis-elements in the following table can be ordered from SyngenTech.

基因元件 gRNA表达载体

Contact us at 4006803200,or send an email to service@syngen.tech Your questions and requests will be answered by expert staff in SyngenTech within 24 hours.