Genetically engineered cell lines are useful tools for studying gene function, tumor treatment, biopharmaceutical research and many other applications. However, the difficult and time-consuming task of generating cell lines can sometimes be a rate-limiting step for a promising project. SyngenTech provides one-stop service for cell line engineering using CRISPR/Cas9 technology. Our featured services include generating knock-out cell lines, site-specific and non-site-specific knock-in cell lines.
1. Knock-out cell lines and site-specific knock-in cell lines
Genome editing technology allows genetic modifications at the genomic DNA level. The CRISPR/Cas9 genome editing system involves expressing an RNA-guided Cas9 endonuclease along with guide RNAs directing it to a specific sequence to be edited. When a DSB (double stranded break) is introduced in the genome, the cell repairs the damage by by NHEJ (Nonhomologous end joining) or HR (Homologous recombination), during which different modifications can be introduced to the genome.：1）Gene knock-out: a particular DSB is introduced to a specific gene locus. Repairing by NHEJ would result in DNA insertion or deletion (Indel), which in turn leads to frame shift.
2. Non-site-specific knock-in cell lines
SyngenTech uses lentivirus as a carrier for delivering of exogenous DNA into genomes. Lentivirus is an HIV-1-based retrovirus that effectively transduces both dividing and non-dividing mammalian cells. Widely used in cell engineering, Lentivirus transduction provides stable, long-term expression of target genes in a wide range of host cells. Compared to other retroviral system, lentivirus vectors results in a higher viral titer and efficient expression of the gene of interest.
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3. Our advantages
Gene knock-out 2-4 months
Site-specific gene knock-in 3-6 months
Non site-specific gene knock-in 1-2 months
5. Payment and result submission
Contact us at 4006803200，or send an email to firstname.lastname@example.org Your questions and requests will be answered by expert staff in SyngenTech within 24 hours.