Cell Line Engineering

Genetically engineered cell lines are useful tools for studying gene function, tumor treatment, biopharmaceutical research and many other applications. However, the difficult and time-consuming task of generating cell lines can sometimes be a rate-limiting step for a promising project. SyngenTech provides one-stop service for cell line engineering using CRISPR/Cas9 technology. Our featured services include generating knock-out cell lines, site-specific and non-site-specific knock-in cell lines.

1. Knock-out cell lines and site-specific knock-in cell lines

Genome editing technology allows genetic modifications at the genomic DNA level. The CRISPR/Cas9 genome editing system involves expressing an RNA-guided Cas9 endonuclease along with guide RNAs directing it to a specific sequence to be edited. When a DSB (double stranded break) is introduced in the genome, the cell repairs the damage by by NHEJ (Nonhomologous end joining) or HR (Homologous recombination), during which different modifications can be introduced to the genome.:

1)Gene knock-out: a particular DSB is introduced to a specific gene locus. Repairing by NHEJ would result in DNA insertion or deletion (Indel), which in turn leads to frame shift.
过程 2)Site-specific gene knock-in: except for the components used by gene knock-out, gene knock-in requires an exogenous donor DNA which offers homologous sequences for the HR repairing.
定点基因敲入 过程

2. Non-site-specific knock-in cell lines

SyngenTech uses lentivirus as a carrier for delivering of exogenous DNA into genomes. Lentivirus is an HIV-1-based retrovirus that effectively transduces both dividing and non-dividing mammalian cells. Widely used in cell engineering, Lentivirus transduction provides stable, long-term expression of target genes in a wide range of host cells. Compared to other retroviral system, lentivirus vectors results in a higher viral titer and efficient expression of the gene of interest.

Technical flowchart:


For ordering, please download and fill in the online form and send to info@syngentech.com.cn Or you can directly contact the marketing department at sales@syngentech.com.cn. We will reach you as soon as possible.

3. Our advantages

4. Timelines

Gene knock-out    2-4 months

Site-specific gene knock-in   3-6 months

Non site-specific gene knock-in   1-2 months

5. Payment and result submission

  1. One-off payment: the client would enjoy 10% discount;
  2. Installment payment: the client should pay 60% of the total charge as a deposit.
  3. The client will receive 2 cell lines and other test reports including sequencing results and RT-PCR results.

Contact us at 4006803200,or send an email to service@syngen.tech Your questions and requests will be answered by expert staff in SyngenTech within 24 hours.